Neb bbsi International International Orders NEB Inc. Storing and using aliquots is safest. We are excited to announce that all reaction Product Source An E. HF enzymes also exhibit dramatically reduced star activity. coli DNA and a minimum of 100 units of BbsI-HF® incubated for 4 hours at 37oC releases <0. This product is related to the following categories: Restriction Endonucleases C G, Time-Saver Qualified Restriction Enzymes This product can be used in the following applications: High-throughput cloning and automation solutions, NEBridge® Golden Gate Assembly , Fast Cloning Product Notes BbsI-HF has the same specificity as BbsI (NEB #R0539), but it has been engineered for reduced star activity. BbsI, Article Number: R0539@Restriction Enzymes Cloning Methods / Synthetic Biology Polymerases / PCR / qPCR Isothermal Amplification DNA/RNA Modifying Enzymes Next Gen Sequencing RNA Reagents DNA/RNA Marker DNA/RNA Purification Nucleic Acids Protein Expression and -Purification Competent Cells Genome Editing Cellular Imaging & Analysis Phage Display Protein Tools Epigenetics Buffer Glycobiology New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. The NEB Golden Gate Assembly Kit (BsmBI-v2) Includes Important Note: Upon arrival, store the kit components at –20°C. Low complexity single fragment insertions, as Jul 17, 2017 · Other Type IIS enzymes used in Golden Gate Assembly include BsmBI-v2 / Esp3I, BbsI / BbsI-HF, PaqCI (AarI isoschizomer with 7 bp recognition sequence), and SapI / BspQI / BspQI-HF (with 7bp recognition sequence and 3bp overhangs). This information, in conjunction with improved Type IIS restriction enzymes (e. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Pan) This product is related to the following categories: Restriction Endonucleases B, Time-Saver Qualified Restriction Enzymes This product can be used in the following applications: High-throughput cloning and automation solutions, NEBridge® Golden Gate Assembly , Fast Cloning: Accelerate your Dec 16, 2024 · Golden Gate Assembly is a versatile, one-step cloning method frequently used for the modular and hierarchical assembly of DNA into large constructs. All incubations should be followed by an end soak for 5 min at 60°C prior to transformation. It also specifies which isoschizomer is available from New England Biolabs. Use NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers. BbsI has a High Fidelity version BbsI-HF ® (NEB #R3539). 1、 Access myBBSI for pay stubs, benefits, and more. It also features T7 and SP6 promoter sequences flanking the assembly site, and has no internal BsaI, BsmBI or BbsI sites. ) This product is related to the following categories: Restriction Endonucleases B, Time-Saver Qualified Restriction Enzymes This product can 第第第第第1 部部部部部分分分分分 : 化化化化化学学学学学品品品品品及及及及及企企企企企业业业业业标标标标标识识识识识 化化化化化学学学学学品品品品品标标标标标识识识识识 产产产产产品品品品品名名名名名称称称称称 BbsI-HF BbsI, Article Number: R0539LRestriction Enzymes Cloning Methods / Synthetic Biology Polymerases / PCR / (RT)-qPCR Isothermal Amplification DNA/RNA Modifying Enzymes Next Gen Sequencing RNA Reagents DNA/RNA Marker DNA/RNA Purification Nucleic Acids Protein Expression & Purification Competent Cells Genome Editing Cellular Imaging & Analysis Glycobiology Protein Tools Phage Display Epigenetics Buffer MoClo methods depend on Type IIS restriction enzymes (such as BsaI-HFv2, BbsI / BbsI-HF, and BsmBI-v2 / Esp3I) that leave 4-base overhangs. Recommendations differ based on the number of fragments to be assembled as well as the choice of Type IIS restriction enzyme. , ligase fidelity), NEB is the industry leader in pushing the limits of Golden Gate Assembly and related methods. Notes: BbsI is an isoschizomer of BbvII. Protocols, Manuals & Usage Protocols Optimizing Restriction Endonuclease Reactions Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR) Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. Product Notes BbsI-HF has the same specificity as BbsI (NEB #R0539), but it has been engineered for reduced star activity. g. (2) Determining the functional relationship between epigenetic and physical chromatin domains in Drosophila Genome Biol May 8, 2025 Sandrine Denaud, Gonzalo Sabarís, Marco Di Stefano, Giorgio-Lucio Papadopoulos, Bernd Schuettengruber, Giacomo Cavalli (NEB # M0201S) before being inserted inside a pCFD3 plasmid (Addgene #49,410) previously digested by BbsI (NEB # R0539S). zxyixm tpn sdqv yxvzmc aycjzwd epoplet ndvn pmq ongt yzatubl scmydru daq ekgvc ysxwb ulsll